Drug-Resistant Tuberculosis:
A Genetic Analysis Using Online Bioinformatics Tools





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Lesson PDF

Background

Materials

Sequences

Questions

Part A

Part B

Part C

Part D

Part E

Websites

Clustal W

GeneMarkS

Copymasters



Part B: Translating DNA into Amino Acids

Thus far in class, you have been transcribing DNA into RNA, identifying codons, then using the genetic code chart to determine the amino acids that are coded for by each codon. You will not need to do transcription and translation of your DNA sequences by hand!! There are websites that biologists use that will translate a DNA sequence into an amino acid sequence.

Steps 8-10) You will now translate each gene sequence into a polypeptide (amino acid sequence)..

a. Go to the website for GeneMarkS. at
http://exon.gatech.edu/GeneMark/genemarks.cgi

b. Go back to the original data file of your wild-type and variant gene sequences.

c. Copy and paste both gene sequences into the large box. Be sure to include the sequence labels again.

d. Under “Output options,” mark the boxes “Protein sequence” and “Gene nucleotide sequence.”

e. Click “Start GeneMarkS.”

f. On the next page, click on the “gms.out.faa” link to retrieve protein sequences.

g. Copy both amino acid sequences.

tableB

The amino acid sequence is given with single-letter codes for the 20 different amino acids. For example, M = Met = Methionine; K = Lys = Lysine.




Link to Part C



Copyright © 2013 Michael Strong and Jessica Taylor